X-Chromosome Inactivation

This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.

Aceasta carte detaliaza tehnologiile dezvoltate recent si procedurile citologice utilizate in mod conventional pentru studiul inactivarii cromozomului X. Capitolele detaliaza imagistica in direct, metodele bioinformatice, hibridizarea in situ a fluorescentei si imunofluorescenta si procedurile pentru optimizarea studiului mecanismului molecular care sta la baza inactivarii cromozomului X.