This book encompasses broad aspects of
Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for
in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
04
02
1.
Cryptosporidium
Diagnostic Assays: MicroscopyGuy Robinson and Rachel M. Chalmers
2.
Cryptosporidium Diagnostic Assays: Molecular Detection
Guy Robinson, Kristin Elwin, and Rachel M. Chalmers
3. Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End UltrafiltrationAmy M. Kahler and Vincent R. Hill
4. Cryptosporidium Oocyst Purification Using Discontinuous Gradient CentrifugationMichael J. Arrowood
5. Measuring Cryptosporidium Serologic Responses by Multiplex Bead AssayJeffrey W. Priest and Delynn M. Moss
6. Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondiiIwona Driskell and Roberta M. O’Connor
7. Cryptosporidium Genotyping for Epidemiology TrackingDawn M. Roellig and Lihua Xiao
8. Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and DetectionAnson V. Koehler and Jan Šlapeta
9. Preparation of Cryptosporidium DNA for Whole Genome SequencingGuy Robinson and Rachel M. Chalmers
10. Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.orgSusanne Warrenfeltz and Jessica C. Kissinger on behalf of the EuPathDB Team
11. A Novel Method to Silence Genes in
CryptosporidiumAlejandro Castellanos-Gonzalez
12. Use of miRNAs to Study Host Cell-Parasite InteractionsJi-Hao Xu, Nicholas W. Mathy, and Xian-Ming Chen
13. Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, and Boris Striepen
14. Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and HistopathologyKarine Sonzogni-Desautels, Jan R. Mead, and Momar Ndao
15. Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of TherapeuticsMichael W. Riggs and Deborah A. Schaefer
16. Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in WaterJennifer L. Murphy and Michael J. Arrowood
17. High-Content Screening for Cryptosporidium Drug DiscoveryMelissa S. Love and Case W. McNamara
18. High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCRHaili Zhang and Guan Zhu
19. In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test CompoundsNigel Yarlett, Mary Morada, Mohini Gobin, Wesley Van Voorhis, and Samuel Arnold
20. In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial MonolayersGeorgia Wilke, Yi Wang, Soumya Ravindran, Thaddeus Stappenbeck, William Witola, and L. David Sibley
21. Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for CryptosporidiumDaviel Cardenas, Seema Bhalchandra, Hymlaire Lamisere, Ying Chen, Xi-Lei Zeng, Sasirekha Ramani, Umesh C. Karandikar, David L. Kaplan, Mary K. Estes, and Honorine D. Ward
18
02
This book encompasses broad aspects of
Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for
in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
19
02
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
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