Laboratory Guide to Two-Dimensional Gel Electrophoresis

Theoretically capable of separating tens of thousands of proteins, two-dimensional gel electrophoresis (2DGE) is often still regarded as procedurally complex and poorly reproducible. An indispensible tool that enables even inexperienced researchers to obtain reliable, highly reproducible separations of protein by 2DGE, this "pocket guide" is a valuable resource for detailed protocols, professional "secrets for success," buffer recipes, and troubleshooting guides that reflect the recognized expertise and years of experience of the author. Multiple illustrations supplement the step-by-step instructions and valuable footnotes found on each page provide instant access to supplementary information.

Teoretic capabil sa separe zeci de mii de proteine, electroforeza bidimensionala pe gel (2DGE) este adesea considerata ca fiind complexa din punct de vedere procesual si slab reproductibila. Un instrument indispensabil care permite chiar si cercetatorilor neexperimentati sa obtina separari fiabile, foarte reproductibile de proteine prin 2DGE, acest „ghid de buzunar” este o resursa valoroasa pentru protocoale detaliate, „secrete profesionale pentru succes”, retete tampon si ghiduri de depanare care reflecta expertiza si ani de experienta a autorului.