Metabolic Network Reconstruction and Modeling

This volume looks at the latest methodologies used to study cellular metabolism with in silico approaches. The chapters in this book are divided into 3 parts: part I discusses tools and methods used for metabolic reconstructions and basic constraint-based metabolic modeling (CBMM); Part II explores protocols for the generation of experimental data for metabolic reconstruction and modeling, including transcriptomics, proteomics, and mutant generations; and Part III cover advanced techniques for quantitative modeling of cellular metabolism, including dynamic Flux Balance Analysis and multi-objective optimization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Metabolic Network Reconstruction and Modeling: Methods and Protocols is a valuable resource for qualified investigators studying cellular metabolism, and novice researchers who want to start working with CBMM.

Acest volum analizeaza cele mai noi metodologii utilizate pentru a studia metabolismul celular cu abordari in silico. Capitolele acestei carti sunt impartite in 3 parti: partea I discuta instrumentele si metodele utilizate pentru reconstructiile metabolice si modelarea metabolica bazata pe constrangeri (CBMM); Partea a II-a exploreaza protocoale pentru generarea de date experimentale pentru reconstructie si modelare metabolica, inclusiv transcriptomica, proteomica si generatii mutante; si partea III acopera tehnici avansate pentru modelarea cantitativa a metabolismului celular, inclusiv analiza dinamica a echilibrului fluxului si optimizarea multi-obiectiva.